Production , purification and characterization of Lipase isolated from salt water Halobacterium sp

Halobacterium are salt loving microorganism, which thrives in the salt lake, and seawater where there is an elevated salt concentration. They have the capacity to balance the osmotic effect of the outside environment and resist against the dehydrating effects of the salts. Their novel characteristics and capacity for large scale culturing make Halobacterium potentially valuable for biotechnology. In the present study the Halobacterium sp. was isolated from seawater sample and from paddy field nearer to seashore they are characterized by morphological and biochemical characteristics. The Halobacterium sp. was subjected to screening for enzyme production like amylase, cellulose, lipase and protease. Lipase enzyme was produced in the laboratory scale it was purified by dialysis method and using ion exchange chromatography. The enzyme activity and concentration were determined using the standard laboratory protocols. The enzyme kinetics studies were also carried out along with the SDS-PAGE using BSA as a standard marker and it was found out that the obtained lipase enzyme was 66 KD in its molecular weight.